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Validation of key protein and methylation changes in epididymal fat. (A) WB detect results of SIRT1, <t>TP53,</t> and BCL2 of epididymal fat. (B) mRNA levels of SIRT1, TP53, and BCL2 of epididymal fat. (C) MSP detection results of epididymal fat SIRT1; A1–A3: NC group, B1–B3: HFD group, C1–C3: HFH group. M, methylated; U, unmethylated. (D) BSP detection results of epididymal fat SIRT1, black color: methylated, white color: unmethylated. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Image Search Results


Representative H&E or IHC images of EEC/AEH. Representative H&E staining of AEH (A) and EEC (B). MSH2 positive staining (C), MSH2 loss (D), p53 abnormal (p53abn) (E), p53 wild-type (F). Abbreviations: AEH: Atypical endometrial hyperplasia; EEC: Endometrioid endometrial cancer; HE: hematoxylin and eosin.

Journal: Biomolecules and Biomedicine

Article Title: Molecular classification and fertility-sparing outcomes in endometrial cancer and atypical endometrial hyperplasia

doi: 10.17305/bb.2025.12445

Figure Lengend Snippet: Representative H&E or IHC images of EEC/AEH. Representative H&E staining of AEH (A) and EEC (B). MSH2 positive staining (C), MSH2 loss (D), p53 abnormal (p53abn) (E), p53 wild-type (F). Abbreviations: AEH: Atypical endometrial hyperplasia; EEC: Endometrioid endometrial cancer; HE: hematoxylin and eosin.

Article Snippet: Conversely, staining of nuclei from both tumor and normal tissue cells was deemed a positive outcome. p53 protein assays were performed using the same IHC method with a p53 primary antibody (1:250; ProteinTech Group).

Techniques: Staining

FA intake impacts on striatal (a) p53, (b) Bcl-2 content, and (c) caspase-3 IHC in rats subjected to 3NP. Data are presented as mean ± SD; ns (non-significant, P > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Ferulic acid mitigates 3-Nitropropionic acid-induced Huntington’s disease via modulation of Nrf2/HO-1, TLR4/NF-κB, and SIRT1/p53 signaling pathways

doi: 10.3389/fphar.2025.1678724

Figure Lengend Snippet: FA intake impacts on striatal (a) p53, (b) Bcl-2 content, and (c) caspase-3 IHC in rats subjected to 3NP. Data are presented as mean ± SD; ns (non-significant, P > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Article Snippet: Striatal p53 (CSB-E08336r) and TNF-α (CSB- E11987r) were assessed using Cusabio kits (Wuhan, China).

Techniques:

Validation of key protein and methylation changes in epididymal fat. (A) WB detect results of SIRT1, TP53, and BCL2 of epididymal fat. (B) mRNA levels of SIRT1, TP53, and BCL2 of epididymal fat. (C) MSP detection results of epididymal fat SIRT1; A1–A3: NC group, B1–B3: HFD group, C1–C3: HFH group. M, methylated; U, unmethylated. (D) BSP detection results of epididymal fat SIRT1, black color: methylated, white color: unmethylated. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Food Science & Nutrition

Article Title: Flavonoids in Rosa roxburghii Tratt Fermentation Broth Ameliorate Obesity via DNMT3a / SIRT1 ‐Mediated Epigenetic Modulation

doi: 10.1002/fsn3.70892

Figure Lengend Snippet: Validation of key protein and methylation changes in epididymal fat. (A) WB detect results of SIRT1, TP53, and BCL2 of epididymal fat. (B) mRNA levels of SIRT1, TP53, and BCL2 of epididymal fat. (C) MSP detection results of epididymal fat SIRT1; A1–A3: NC group, B1–B3: HFD group, C1–C3: HFH group. M, methylated; U, unmethylated. (D) BSP detection results of epididymal fat SIRT1, black color: methylated, white color: unmethylated. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies targeting TNF‐α (60291‐1‐Ig), SIRT1 (13161‐1‐AP), tumor protein P53 (TP53, 60283‐2‐Ig), hypoxia‐inducible factor 1‐alpha (HIF1a, 66730‐1‐Ig), nuclear factor‐kappa B (NF‐κB, 10745‐1‐AP) and PPARγ (16643‐1‐AP) from Proteintech (Wuhan, China), as well as IL6 (TD6087) and B‐cell lymphoma 2 (BCL2, T40056 ) from Abmart (Shanghai, China).

Techniques: Biomarker Discovery, Methylation